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Immunohistochemical analysis of Serpin A8/Angiotensinogen was detected in perfusion fixed frozen sections of mouse intestine using Rat Anti-mouse Serpin A8/Angiotensinogen Monoclonal Antibody (Product # MA5-24361) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB (brown and counterstained with hematoxylin (blue). Specific staining was localized to intestinal epithelial cells.
|Tested species reactivity||Mouse|
|Host / Isotype||Rat / IgG1|
|Immunogen||CHO-derived recombinant mouse Serpin A8/Angiotensinogen Asp25-Val477|
|Storage buffer||PBS with 5% trehalose|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||8-25 µg/ml|
|Western Blot (WB)||0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
AGT; Angiotensin I; Angiotensin II; angiotensin ll; angiotensinogen; ANHU; Pre-angiotensinogen; serpin A8; SERPINA8
AI265500; AngI; AngII; Aogen; Serpina8