|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant human IL-16|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
M160E targets IL-16 in ELISA, and WB applications and shows reactivity with Human samples.
The M160E immunogen is recombinant human IL-16.
M160E detects IL-16 which has a predicted molecular weight of approximately 142 kDa.
The M160E IL16 antibody (clone 17.1) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M161B (biotinylated conjugate of clone 14.1) Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Antibody M160E (clone 17.1) and biotinylated antibody M161B (clone 14.1) have successfully been used in combination with recombinant IL16 protein SIL16 in ELISA applications.
The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Phenotypic differences between Th1 and Th17 cells and negative regulation of Th1 cell differentiation by IL-17.
M160E was used in flow cytometry to characterize and compare T helper 1 and 17 cells
|Nakae S,Iwakura Y,Suto H,Galli SJ||Journal of leukocyte biology (81:1258)||2007|