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Western blot analysis from lysates of mouse brain (cortex) tissue and mouse brain stem tissue, loaded at 0.2 mg/mL. A specific band was detected for CHL-1/L1CAM-2 at approximately 196-201 kDa (as indicated) using 2.5 µg/mL of Goat Anti-mouse CHL-1/L1CAM-2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47259)
|Tested species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Mouse myeloma cell line NS0-derived recombinant mouse CHL-1/L1CAM-2 Ala25-Gln1043 (Leu227-Gln242 del, Ala243Ser)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 5% trehalose|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||5-15 µg/ml|
|Western Blot (WB)||0.25-2.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human CHL-1 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
The protein encoded by this gene is a member of the L1 gene family of neural cell adhesion molecules. It is a neural recognition molecule that may be involved in signal transduction pathways. The deletion of one copy of this gene may be responsible for mental defects in patients with 3p- syndrome. Several alternatively spliced transcript variants of this gene have been described, but their full length nature is not known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
chl1-like protein, close homolog of L1, neural cell adhesion molecule L1-like protein, CALL, L1CAM2, L1 cell adhesion molecule 2, cell adhesion molecule with homology to L1CAM (close homologue of L1)
CALL, LICAM2, AI465420, A530023M13Rik