Western blot analysis of OCT1/OTF1 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) NIH 3T3 cell lysate using a OCT1/OTF1 polyclonal antibody (Product # PA5-23374) at 2 ug/ml, 2 ug/ml and 4 ug/ml respectively. Goat anti-rabbit Ig HRP secondary antibody.
|Tested species reactivity||Bovine, Horse, Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (428 CNRRQKEKRINPPSSGGTS 446) of OCT1/OTF1 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||2-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
OCT1/OTF1 is a protein belonging to the POU domain of transcription factors. OCT1, Octamer binding transcription factor 1, binds to an octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes for immunoglobulins. POU domain proteins contain a bipartite DNA-binding domain, which is divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is conferred at the dimerization level. POU dimer from the OCT1 gene formed on the palindromic OCT factor recognition element, or PORE, could recruit the transcriptional coactivator OBF1. This octamer sequence within immunoglobulin promoters are sufficient for lymphoid-specific promoter activity in lymphoid cells. OCT1 regulate the expansion and/or maintenance of mature B cells. In its absence mature B-cell pull is strongly reduced.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.