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|Tested species reactivity||Mouse , Zebra fish , Dog , Hamster , Human , Porcine , Rat , Primate , Eubacteria , Cattle|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide made to an N-terminal portion of the human LC3 protein sequence (between residues 1-100).|
|Purification||Antigen affinity chromatography|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200-1:400|
|Immunoprecipitation (IP)||20 µg/500 µg of protein|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody has 92% homology with mouse, 91% with bovine, and 84% with xenopus.
Reacts weaker with mouse than with human samples
LC3, a mammalian homologue of Apg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. However, LC3 is now thought to also be involved in autophagy. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. LC3-II is the first mammalian protein identified that specifically associates with the autophagosome membranes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
MAP1 light chain 3-like protein 2, MAP1A/1B light chain 3 B, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, autophagy-related protein LC3 B, autophagy-related ubiquitin-like modifier LC3 B, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated proteins 1A/1B light chain 3B, RP11-346K17.1, ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, autophagy-related protein LC3 A, autophagy-related ubiquitin-like modifier LC3 A, microtubule-associated proteins 1A/1B light chain 3A
MAP1LC3B-a, LC3B, LC3b, zbs559, ATG8F, Atg8, 1010001C15Rik, MAP1A/1BLC3, Mpl3, Map1lc3