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Immunohistochemical analysis of Hepassocin was detected in perfusion fixed frozen sections of rat liver using 15 µg/mL Goat Anti-Rat Hepassocin Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47068) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB (brown and counterstained with hematoxylin (blue).
|Tested species reactivity||Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||Mouse myeloma cell line NS0-derived recombinant rat Hepassocin Asp25-Val314|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 5% trehalose|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||5-15 µg/ml|
|Western Blot (WB)||0.1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Reconstitute at 0.2 mg/mL in sterile PBS.
Fibrinogen-like 1 is a member of the fibrinogen family. This protein is homologous to the carboxy terminus of the fibrinogen beta- and gamma- subunits which contains the four conserved cysteines of fibrinogens and fibrinogen related proteins. However, this protein lacks the platelet-binding site, cross-linking region and a thrombin-sensitive site which are necessary for fibrin clot formation. This protein may play a role in the development of hepatocellular carcinomas. Four alternatively spliced transcript variants encoding the same protein exist for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
fibrinogen-like protein 1, fibrinogen-related protein 1, fibronigen-like protein 1, liver fibrinogen-related protein 1, HFREP1, LFIRE1, LFIRE-1, hepassocin, hepatocellular carcinoma-related sequence, hepatocyte-derived fibrinogen-related protein 1