|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 16 amino acid peptide near the center of human ZIP3.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||5 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control is mouse small intestine tissue lysate.
PA5-21068 can be used with blocking peptide PEP-1182.
The zinc transporter ZIP3, also known as SLC39A3, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. Similar to knock-outs of ZIP1 and ZIP2, ZIP3-null mice have no phenotypic differences compared to wild-type mice. Only when ZIP1, ZIP2, and ZIP3 genes are all eliminated and these mutant mice are fed a zinc-deficient diet do abnormalities such as reduced embryonic-membrane bound alkaline phosphatase activity and abnormal development occur, indicating that the ZIP1-3 proteins play an important, noncompensatory role when zinc is deficient. More recent studies have shown that ZIP2 and ZIP3 are down regulated in human prostate adenocarcinomatous glands, and may be important in the retention of zinc in the cellular compartment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.