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|Tested species reactivity||Primate , Human , Mouse , Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from human EGFR around the phosphorylation site of Threonine 678|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Concentration is lot-specific and will vary from 0.5-0.6 mg/ml
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, epidermal growth factor receptor, waved 2, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, proto-oncogene c-ErbB-1, receptor tyrosine-protein kinase erbB-1, EC 18.104.22.168, ERBB1, Epidermal growth factor receptor precursor, Receptor protein-tyrosine kinase ErbB-1, kinase EGFR
PIG61, wa2, Errp, Wa5, Erbb, ERBB, AI552599, 9030024J15Rik, Errb1, ERBB1, wa-2, mENA, HER1
Protein metabolism and modification Protein modification Protein phosphorylation Signal transduction Cell surface receptor mediated signal transduction Receptor protein tyrosine kinase signaling pathway Oncogenesis Developmental processes Gametogenesis Oogenesis Embryogenesis Determination of dorsal/ventral axis Cell cycle Cell cycle control Apoptosis Inhibition of apoptosis Cell proliferation and differentiation