Immunohistochemical staining of paraformaldehyde-fixed paraffin-embedded human cerebellum sections at 400x magnification with DAPI counterstain. Primary antibody used was a SEPT5/CDCre-1 monoclonal antibody (clone SP18) at a 1:500 dilution, followed by secondary antibody was a 488 fluorescent conjugated Goat anti-mouse Ig at a 1:500 dilution and Normal Goat Serum (blocking)
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17618 targets SEPT5/CDCre-1 in ELISA, FACS, and WB applications and shows reactivity with Human samples.
The MA5-17618 immunogen is human CDCrel-1/SEPT5.
This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Two transcripts of this gene, a major one of 2.2 kb and a minor one of 3.5 kb, have been observed. The 2.2 kb form results from the utilization of a non-consensus polyA signal (AACAAT). In the absence of polyadenylation from this imperfect site, the consensus polyA signal of the downstream neighboring gene (GP1BB; platelet glycoprotein Ib) is used, resulting in the 3.5 kb transcript. An alternatively spliced transcript variant with a different 5' end has also been identified, but its full-length nature has not been completely determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.