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Immunohistochemical analysis of IL-15 Ra was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 15 µg/mL Goat Anti-human IL-15 Ra Antigen Affinity-purified Polyclonal Antibody (Product # PA5-46991) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB (brown and counterstained with hematoxylin (blue).
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Sf 21-derived recombinant human IL-15 R alpha Ile31-Thr172|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 5% trehalose|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||2.5 µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||5-15 µg/ml|
|Neutralization (Neu)||1-4 µg/ml|
|Western Blot (WB)||0.1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In direct ELISAs, less than 10% cross-reactivity with recombinant human IL-2 R gamma is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Interleukin 15 (IL-15RA) is a cytokine receptor that specifically binds IL-15 with high affinity. IL-15 regulates T and natural killer cell activation and proliferation. The IL-15 and IL-2 receptors share two subunits, IL-2R beta and IL-2R gamma, and IL-15RA is structurally related to IL-2RA, an additional IL-2-specific alpha subunit necessary for high affinity IL-2 binding. Unlike IL-2RA, IL-15RA is capable of binding IL-15 with high affinity independent of the other subunits. IL-15RA is thought to enhance cell proliferation and expression of apoptosis inhibitor Bcl-xL and Bcl-2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
interleukin-15 receptor subunit alpha, IL-15RA, Interleukin-15 receptor subunit alpha, IL15RA, CD215