Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunocytochemical analysis of MafF was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-human MafF Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47552) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-Goat IgG Secondary Antibody (left panel, re and counterstained with DAPI (right panel, blue). Specific staining was localized to nuclei.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||E. coli-derived recombinant human MafF Ser2-Ser164|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 5% trehalose|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||5-15 µg/ml|
|Immunofluorescence (IF)||5-15 µg/ml|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In Western blots, less than 1% cross-reactivity with recombinant human (rh) MafG and rhMafK is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. The encoded protein can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. This gene may also be involved in the cellular stress response. Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
hMafF; U-MAF; v-maf avian musculoaponeurotic fibrosarcoma oncogene family protein F; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog F; v-maf musculoaponeurotic fibrosarcoma oncogene homolog F avian
hMafF; MAFF; U-MAF