PKC-beta II [pT641] phosphospecific antibody. PMA-treated K652 cell lysates were incubated with anti-PKC-beta II [pT641] after prior incubation with: no peptide (1, 2), the non-phosphopeptide (3), a generic [pT] peptide (4), or, the phosphopeptide immunogen (5). Peptide competition data show antibody phospho-site specificity. Lane 1 was treated with l phosphatase prior to immunoblot. Absence of signal further demonstrates antibody specificity.
|Tested species reactivity||Human|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PKCbII that contains threonine 641. The sequence is conserved in mouse and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
||Demonstration of the essential role of protein kinase C isoforms in hyperglycemia-induced embryonic malformations.||Wu YK,Reece EA||Reproductive sciences (Thousand Oaks, Calif.) (15:349)||2008|
|Rat||Not Cited||Demonstration of the essential role of protein kinase C isoforms in hyperglycemia-induced embryonic malformations.||Wu YK,Reece EA||Reproductive sciences (Thousand Oaks, Calif.) (15:349)||2008|