|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Mouse CD86 (B7-2)|
|Storage buffer||PBS with 4-5mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1.0 ug/10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17954 targets CD86/B7-2 in FACS applications and shows reactivity with Mouse samples.
The MA5-17954 immunogen is mouse CD86 (B7-2).
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Conventional bone marrow-derived dendritic cells contribute to toll-like receptor-independent production of alpha/beta interferon in response to inactivated parapoxvirus ovis.
MA5-17954 was used in flow cytometry to study the effect of inactivated Parapoxvirus ovis on dendritic cells
|Siegemund S,Hartl A,von Buttlar H,Dautel F,Raue R,Freudenberg MA,Fejer G,Büttner M,Köhler G,Kirschning CJ,Sparwasser T,Alber G||Journal of virology (83:9411)||2009|
Passive transfer of flt-3L-derived dendritic cells delays diabetes development in NOD mice and associates with early production of interleukin (IL)-4 and IL-10 in the spleen of recipient mice.
MA5-17954 was used in flow cytometry to assess the contribution of DC function in non-obese diabetic mice.
|Morin J,Faideau B,Gagnerault MC,Lepault F,Boitard C,Boudaly S||Clinical and experimental immunology (134:388)||2003|
Naive B lymphocytes undergo homeostatic proliferation in response to B cell deficit.
MA5-17954 was used in flow cytometry to investigate homeostatic B cell proliferation.
|Cabatingan MS,Schmidt MR,Sen R,Woodland RT||Journal of immunology (Baltimore, Md. : 1950) (169:6795)||2002|
Dendritic cells in the induction of protective and nonprotective anticryptococcal cell-mediated immune responses.
MA5-17954 was used in flow cytometry to examine the contribution of different DC subsets during infection with Cryptococcus neoformans.
|Bauman SK,Nichols KL,Murphy JW||Journal of immunology (Baltimore, Md. : 1950) (165:158)||2000|