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Western blot analysis from lysates of MT-2 human T cell line and CHO Chinese hamster ovary cell line either mock transfected or transfected with human IL-27 p28/IL-27A. PVDF membrane was probed with 1 µg/mL of Goat Anti-human IL-27 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-46993) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. Specific bands were detected for the IL-27 EBI3/IL-27B at approximately 32 kDa and IL-27 p28/IL-27A at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Mouse myeloma cell line NS0-derived recombinant human IL-27 Arg21-Lys229 (IL-27 EBI-3 subunit), Phe29-Pro243 (IL-27 p28 subunit)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 5% trehalose|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-0.4 µg/ml (Detection), 0.2-0.8 µg/ml (Capture)|
|Neutralization (Neu)||1-5 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In sandwich ELISAs, less than 0.2% cross-reactivity with recombinant mouse IL-27, recombinant human (rh) IL-12, and rhIL-12 p40 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Interleukin 27, or IL-27 is a cytokine belonging to the IL-12 family. It is a heterodimeric cytokine composed of EBI3 and p28 subunits. IL-27 is produced by activated dendritic cells and macrophages in response to Toll-like receptor ligands and pro-inflammatory cytokines. IL-27 promotes CD4+ T cell differentiation to the Th1 lineage by inducing expression of the transcription factor T-bet and up regulating IL-12R beta2, which in turn suppresses Th2 and Th17 differentiation and proliferation. In humans, the gene is located on the p arm of chromosome 16.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
EBV-induced gene 3 protein, Epstein-Barr virus induced gene 3, IL-27 subunit beta, IL27 subunit, IL35 subunit, cytokine receptor, epstein-Barr virus-induced gene 3 protein, interleukin-27 subunit beta, IL-27 p28 subunit, IL-27 subunit alpha, IL-27-A, IL27-A, interleukin 30, interleukin-27 subunit alpha, IL27, EBI3, IL-27A, IL27A, IL27 p28, IL30, Interleukin-27 subunit beta, IL-27B, IL27B
IL27A, IL27B, IL30, IL-27B, IL-27A, p28, IL-27, IL27p28
Signal transduction Cell surface receptor mediated signal transduction Cytokine and chemokine mediated signaling pathway Intracellular signaling cascade JAK-STAT cascade Cell communication Ligand-mediated signaling Developmental processes Ectoderm development Neurogenesis Biological process unclassified