Western blot analysis of phosphoserine/threonine/tyrosine was performed by loading 30ug of HEK293T cells, either untreated (UT, left lane) or treated with alkaline phosphatase (AP, right lane) per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 1 hour at room temperature. The membrane was probed with a phosphoserine/threonine/tyrosine monoclonal antibody (Product # MA1-38450) at a dilution of 1:1000 for overnight at 4°C, washed in TBST, and probed with an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:40,000 for 1 hour at room temperature. A blot was also probed with an antibody against actin to determine equal loading. Chemiluminescent detection was performed using ECL substrate. Data courtesy of the Innovators Program.
|Tested species reactivity||Chemical|
|Published species reactivity||Fruit fly|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Phosphoserine, phosphothreonine, and phosphotyrosine conjugated to protein carriers.|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1 µg/ml|
|Western Blot (WB)||1:50-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA1-38450 detects phosphotyrosine, phosphoserine, and phosphothreonine in WB and ELISA applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Fruit fly||Not Cited||
The MAP kinase ERK and its scaffold protein MP1 interact with the chromatin regulator Corto during Drosophila wing tissue development.
MA1-38450 was used in western blot to investigate the effect of ERK and MP1 on wing development in Drosophilia
|Mouchel-Vielh E,Rougeot J,Decoville M,Peronnet F||BMC developmental biology (11:null)||2011|