|Tested species reactivity||Hamster, Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Multidrug-resistant Chinese hamster ovary cell line (CHrC5).|
|Storage buffer||tissue culture supernatant with 1% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:10|
|Immunohistochemistry (Frozen) (IHC (F))||1:20|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Western Blot (WB)||1:10|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
This antibody reacts with a conserved cytoplasmic epitope of the plasma membrane-associated 170-180 kD glycoprotein, the expression of which is strongly correlated with the degree of multi-drug-resistance (MDR) derived cell lines, including cell lines derived from lung, ovaries and B cell lymphomas. This product cross-reacts with chinese hamster but does not cross-react with mouse or rat.
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Multidrug resistance-related phenotype and apoptosis-related protein expression in ovarian serous carcinomas.
MA1-35604 was used in immunohistochemistry - paraffin section to assess the extent and prognostic significance of MDR-related protein expression in ovarian serous carcinomas and correlate their expression with apoptosis-related proteins
|Yakirevich E,Sabo E,Naroditsky I,Sova Y,Lavie O,Resnick MB||Gynecologic oncology (100:152)||2006|