|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Syrian hamster / IgG|
|Immunogen||Mouse T lymphoma EL-4 cells.|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.25-0.5 ug per 1x10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Regulation of IgE production and airway reactivity by CD4¿CD8¿ regulatory T cells.
HM3500 was used in flow cytometry to characterize high zone tolerance in mouse models of type I allergy and of allergic airway inflammation.
|Raker V,Stein J,Montermann E,Maxeiner J,Taube C,Reske-Kunz AB,Sudowe S||Immunobiology (220:490)||2015|
IL-10 regulates early IL-12-mediated immune responses induced by the radiation-attenuated schistosome vaccine.
HM3500 was used in flow cytometry to investigate how IL-10 regulates IL-12 production in skin treated with radiation-attenuated schistosomes.
|Hogg KG,Kumkate S,Mountford AP||International immunology (15:1451)||2003|
CD8+ tumor-infiltrating lymphocytes are primed for Fas-mediated activation-induced cell death but are not apoptotic in situ.
HM3500 was used in flow cytometry to characterize murine tumor-infiltrating lymphocytes.
|Radoja S,Saio M,Frey AB||Journal of immunology (Baltimore, Md. : 1950) (166:6074)||2001|
Inhibition of T lymphocyte activation in mice heterozygous for loss of the IMPDH II gene.
HM3500 was used in blocking assay to characterize IMPDH II(-/-) and IMPDH II(+/-) mice.
|Gu JJ,Stegmann S,Gathy K,Murray R,Laliberte J,Ayscue L,Mitchell BS||The Journal of clinical investigation (106:599)||2000|
Role of inflammatory infiltrate in activation and effector function of cloned islet reactive nonobese diabetic CD8+ T cells: involvement of a nitric oxide-dependent pathway.
HM3500 was used in blocking assay to study how CD8+ T cells interact with beta cells and local inflammatory cells in islets.
|Gurlo T,Kawamura K,von Grafenstein H||Journal of immunology (Baltimore, Md. : 1950) (163:5770)||1999|
CD8+ T cells become nonresponsive (anergic) following activation in the presence of costimulation.
HM3500 was used in flow cytometry to study activation-induced nonresponsiveness of T cells.
|Deeths MJ,Kedl RM,Mescher MF||Journal of immunology (Baltimore, Md. : 1950) (163:102)||1999|
Effects of dietary docosahexaenoic acid on surface molecules involved in T cell proliferation.
HM3500 was used in flow cytometry to examine the effect of dietary docosahexaenoic acid on the surface molecules involved in T cell proliferation.
|Sasaki T,Kanke Y,Kudoh K,Misawa Y,Shimizu J,Takita T||Biochimica et biophysica acta (1436:519)||1999|
Two types of anti-TL (thymus leukemia) CTL clones with distinct target specificities: differences in cytotoxic mechanisms and accessory molecule requirements.
HM3500 was used in blocking assay to characterize TCRalphabeta CTL clones that recognize mouse thymus leukemia.
|Tsujimura K,Takahashi T,Iwase S,Matsudaira Y,Kaneko Y,Yagita H,Obata Y||Journal of immunology (Baltimore, Md. : 1950) (160:5253)||1998|
Targets of p56(lck) activity in immature thymoblasts: stimulation of the Ras/Raf/MAPK pathway.
HM3500 was used in flow cytometry to demonstrate that the Ras/Raf/MAPK pathway is constitutively activated in lck-transformed immature thymoblasts.
|Lin K,Abraham KM||International immunology (9:291)||1997|
|Not Applicable||Not Cited||
Pretransplant infusion of mesenchymal stem cells prolongs the survival of a semiallogeneic heart transplant through the generation of regulatory T cells.
HM3500 was used in blocking or activating experiment to test if mesenchymal stem cells have immunomodulatory properties in solid organ allotransplantation
|Casiraghi F,Azzollini N,Cassis P,Imberti B,Morigi M,Cugini D,Cavinato RA,Todeschini M,Solini S,Sonzogni A,Perico N,Remuzzi G,Noris M||Journal of immunology (Baltimore, Md. : 1950) (181:3933)||2008|
|Not Applicable||Not Cited||
Cyclin-dependent kinase inhibition limits glomerulonephritis and extends lifespan of mice with systemic lupus.
HM3500 was used in blocking or activating experiment to study the effects of CDK inhibition on glomerulonephritis and lifespan of mice with systemic lupus
|Zoja C,Casiraghi F,Conti S,Corna D,Rottoli D,Cavinato RA,Remuzzi G,Benigni A||Arthritis and rheumatism (56:1629)||2007|