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Western blot analysis of Mouse Brain Tissue Lysate using PA5-19486, N Cadherin primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19486 staining of Rat Brain Tissue Lysate at a dilution of 1 ug/ml (lane 2). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
|Tested species reactivity||Mouse , Porcine , Rat , Clawed frog , Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 800 - 900 of Human N Cadherin.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||10 µg/ml|
|Immunofluorescence (IF)||5 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||1:300|
|Immunohistochemistry (Paraffin) (IHC (P))||1 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat mediated antigen retrieval recommended prior to tissue staining.
This antibody is predicted to react with chicken and cow based on sequence homology.
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
cadherin 2 type 1 N-cadherin (neuronal), cadherin 2, type 1, N-cadherin (neuronal), cadherin-2, neural cadherin, N-cadherin, N-cadherin-2, cadherin 2, type 1, N-cadherin a (neuronal), neural cadherin-2, N-cadherin 1, cadherin 2, N-cadherin (neuronal), calcium-dependent adhesion protein, neuronal, neural-cadherin, Cadherin-2, Neural-cadherin, CDw325 antigen, CD325, CDHN, NCAD
cdh2a, N-cadherin, CDHN, CDw325, CD325, cadherin-2, cdhn-a, NCAD, Ncad