Purified human CD4+ cells were stimulated with immobilized Anti-Human CD3 Functional Grade Purified, soluble Anti-Human CD28 Functional Grade Purified, Human IL-2, and Human IL-4 for 2 days, followed by IL-2 and IL-4 for 3 days, and restimulated with PMA/Ionomycin in the presence Brefeldin A (A18467) for 4 hours. The cells were surface stained with Anti-Human CD4 PE and intracellularly stained with Mouse IgG1 K Isotype Control FITC (top) or Anti-Human IL-13 FITC (bottom, A18467).
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||proprietary buffer|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
IL-13 is a cytokine involved in regulating inflammatory and immune responses. It down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF.
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