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Flow cytometry analysis of CD29 in peripheral blood granulocytes compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and primary antibody (Product # MA2910) at a dilution of 0.5 ug/test. Cells were incubated for 30 min at 4°C and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4°C in the dark. FACS analysis was performed using 400 ul of cell buffer.
|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human Beta1 Integrin, the Beta1 chain of the Beta1 integrin heterodimer (CD29)|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5 ug/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA2910 targets CD29 in FACS applications and shows reactivity with Human samples.
The MA2910 immunogen is human Beta1 Integrin, the Beta1 chain of the Beta1 integrin heterodimer (CD29).
MA2910 detects CD29 which has a predicted molecular weight of approximately 88 kDa.
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Roles of focal adhesions and fibronectin-mediated cohesion in proliferation of confluent fibroblasts.
MA2910 was used in blocking/activating experiment to study the roles of focal adhesion and intercellular cohesion in the proliferation of normal human fibroblasts
|Tanaka M,Abe T,Hara Y||Journal of cellular physiology (219:194)||2009|
PLCgamma1 is essential for early events in integrin signalling required for cell motility.
MA2910 was used in blocking/activating experiment to study the role of PLC gamma 1 in cell motility signalling
|Jones NP,Peak J,Brader S,Eccles SA,Katan M||Journal of cell science (118:2695)||2005|
Collective and single-molecule interactions of alpha5beta1 integrins.
MA2910 was used in blocking/activating experiment to investigate the collective and single-molecule interactions between alpha5beta1 receptor and GRGDSP ligand
|Kokkoli E,Ochsenhirt SE,Tirrell M||Langmuir : the ACS journal of surfaces and colloids (20:2397)||2004|
Syndecan-1-mediated cell spreading requires signaling by alphavbeta3 integrins in human breast carcinoma cells.
MA2910 was used in blocking/activating experiment to study the role of alphavbeta3 integrin in syndecan-1-mediated cell spreading
|Beauvais DM,Rapraeger AC||Experimental cell research (286:219)||2003|
Chemotaxis of non-compressed blood polymorphonuclear leukocytes from an adolescent with severe leukocyte adhesion deficiency.
MA2910 was used in blocking/activating experiment to study the CD18 mutation in a patient with severe leukocyte adhesion deficiency
|Malawista SE,de Boisfleury Chevance A,Brown EJ,Boxer LA,Law SK||American journal of hematology (73:115)||2003|
Actin-rich spherical extrusion induced in okadaic acid-treated K562 cells by crosslinking of membrane microdomains.
MA2910 was used in blocking/activating experiment to study the effect of membrane microdomain crosslinkingon actin-rich spherical extrusion in OKA-treated cells
|Baba T,Udaka K,Terada N,Ueda H,Fujii Y,Ohno S,Sato SB||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (51:245)||2003|
Extracellular matrix regulates induction of alkaline phosphatase expression by ascorbic acid in human fibroblasts.
MA2910 was used in blocking/activating experiment to investigate the role of extracellular matrix for induction of alkaline phosphatase expression by ascorbic acid in human fibroblasts
|Abe T,Abe Y,Aida Y,Hara Y,Maeda K||Journal of cellular physiology (189:144)||2001|
Yersinia enterocolitica invasin protein triggers IL-8 production in epithelial cells via activation of Rel p65-p65 homodimers.
MA2910 was used in blocking/activating experiment to study the interleukin 8 production by HeLa cells incubated with anti-beta1 integrin monoclonal antibody
|Schulte R,Grassl GA,Preger S,Fessele S,Jacobi CA,Schaller M,Nelson PJ,Autenrieth IB||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (14:1471)||2000|
Heterologous desensitization of IL-8-mediated chemotaxis in human neutrophils by a cell-binding fragment of fibronectin.
MA2910 was used in blocking/activating experiment to study the effect of fibronectin fragment on chemotaxis in human neutrophils
|Stanton KJ,Frewin MB,Gudewicz PW||Journal of leukocyte biology (65:515)||1999|
A role for caveolin and the urokinase receptor in integrin-mediated adhesion and signaling.
MA2910 was used in blocking/activating experiment to study integrin-mediated adhesion
|Wei Y,Yang X,Liu Q,Wilkins JA,Chapman HA||The Journal of cell biology (144:1285)||1999|
Adhesion to vascular cell adhesion molecule 1 and fibronectin. Comparison of alpha 4 beta 1 (VLA-4) and alpha 4 beta 7 on the human B cell line JY.
MA2910 was used in blocking/activating experiment to investigate the importance of beta 1 in the functions of integrin VLA-4
|Chan BM,Elices MJ,Murphy E,Hemler ME||The Journal of biological chemistry (267:8366)||1992|
Distribution of costameric proteins in the diaphragm of patients with chronic obstructive pulmonary disease.
MA2910 was used in immunohistochemistry to study the expression of beta 1 integrin in the diaphragm of COPD and non-COPD patients
|Wijnhoven JH,Hafmans T,Dekhuijzen PN||Respiration; international review of thoracic diseases (73:529)||2006|
EGFR is a transducer of the urokinase receptor initiated signal that is required for in vivo growth of a human carcinoma.
MA2910 was used in immunoprecipitation to study the role of EGFR in the signal transduction pathway for human carcinoma growth
|Liu D,Aguirre Ghiso J,Estrada Y,Ossowski L||Cancer cell (1:445)||2002|
The chemokine receptor CXCR4 enhances integrin-mediated in vitro adhesion and facilitates engraftment of leukemic precursor-B cells in the bone marrow.
MA2910 was used in flow cytometry to investigate the role of SDF-1 and CXCR4 in regulation of beta 1 integrin function
|Shen W,Bendall LJ,Gottlieb DJ,Bradstock KF||Experimental hematology (29:1439)||2001|
Influence of adhesion molecule expression by human brain microvessel endothelium on cancer cell adhesion.
MA2910 was used in flow cytometry to study the effect of adhesion molecule expression on cancer cell adhesion
|Brayton J,Qing Z,Hart MN,VanGilder JC,Fabry Z||Journal of neuroimmunology (89:104)||1998|
Adhesion of alpha5beta1 receptors to biomimetic substrates constructed from peptide amphiphiles.
MA2910 was used in ELISA to evaluate the utility of peptide amphiphiles
|Dillo AK,Ochsenhirt SE,McCarthy JB,Fields GB,Tirrell M||Biomaterials (22:1493)||2001|
CD29; Fibronectin receptor subunit beta; FNRB; Glycoprotein IIa; GPIIA; integrin beta 1; Integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA; VLAB
CD29; FNRB; GPIIA; ITGB1; MDF2; MSK12; VLA-BETA; VLAB