Immunohistochemistry analysis of Phospho-Paxillin pSer178 showing staining in the cytoplasm of paraffin-embedded human lung tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- Phospho-Paxillin pSer178 Polyclonal Antibody (441026G) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human paxillin that contains serine 178.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Inhibition of the invasion and migration of renal carcinoma 786¿o¿si3 cells in vitro and in vivo by Koelreuteria formosana extract.
44-1026G was used in western blot to report that Koelreuteria formosana ethanolic extract inhibits the invasion and migration of renal cell carcinoma cells.
|Lin CY,Chen PN,Hsu LS,Kuo DY,Chu SC,Hsieh YS||Molecular medicine reports (10:3334)||2014|
Regulation of neurite growth by inorganic pyrophosphatase 1 via JNK dephosphorylation.
44-1026G was used in western blot to assess the role of pyrophosphatase in neuronal differentiation.
|Tezuka Y,Okada M,Tada Y,Yamauchi J,Nishigori H,Sanbe A||PloS one (8:null)||2013|
|Human||Not Cited||c-Jun N-terminal kinase is necessary for platelet-derived growth factor-mediated chemotaxis in primary fibroblasts.||Amagasaki K,Kaneto H,Heldin CH,Lennartsson J||The Journal of biological chemistry (281:22173)||2006|
||c-Jun N-terminal kinase is necessary for platelet-derived growth factor-mediated chemotaxis in primary fibroblasts.||Amagasaki K,Kaneto H,Heldin CH,Lennartsson J||The Journal of biological chemistry (281:22173)||2006|