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|Tested species reactivity||Not Applicable|
|Host / Isotype||Yeast|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Control (Ctrl)||Assay Dependent|
|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The mature, soluble form of porcine TNFa was expressed in Pichia pastoris, KM71H. GenBank Accession # P23563, amino acids 77 - 232. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: LRSSSQTSDK. Purity is >95% by SDS-PAGE analyses.
Activity: The biological activity of recombinant porcine TNFa was measured by its cytolytic effect on porcine PK(15) cells in the presence of Actinomycin D. The ED50 (or 1 unit of activity) for this effect typically falls into the range of 7 - 18 pg/ml.
Use: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments.
Reconstitution and Dilution: Store lyophilized material at 4°C. Reconstitute in 0.5 ml of distilled or deionized water. Dilute in a buffer-containing carrier protein, aliquot, and store at or below -70°C, avoiding multiple freeze-thaw cycles. PBS with 1% BSA or a neutral media containing <10% FCS are suitable buffers.
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
cachectin; TNF alpha; TNF-a; TNF-alpha; tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha; tumor-necrosis factor
Signal transduction Cell surface receptor mediated signal transduction Cytokine and chemokine mediated signaling pathway Cell communication Ligand-mediated signaling Immunity and defense Apoptosis Induction of apoptosis