|Tested species reactivity||Dog|
|Host / Isotype||Yeast|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For dilution, use a buffer containing carrier protein, e.g. PBS with 1% BSA or a neutral media containing 10% FCS and freeze in aliquots at -70°C.
Activity: The biological activity of recombinant canine TNFa was measured by its cytolytic effect on mouse WEHI-13VAR cells in the presence of Actinomycin D. The ED50 (or 1 unit of activity) for this effect in our laboratories is typically 10 pg/ml. Refs: Kitani, H. et al. (2002) Infect. Immun. 70:2210. Khabar, K.S. et al. (1995) Immunol. Lett. 46:107.
Expression: The mature, soluble form of canine TNF-alpha was expressed in Pichia pastoris, X-33. GenBank® Accession # P51742, amino acids 77 - 233. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: VKSSSRTPSD.
Reconstitute with 0.5 ml of distilled water.
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.