|Tested species reactivity||Mouse|
|Published species reactivity||Rat, Fish, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant mouse TNF alpha|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Inhibition Assays (IA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
P350 targets TNF Alpha in ELISA, IA, and WB applications and shows reactivity with mouse samples.
The P350 immunogen is recombinant mouse TNF alpha.
P350 detects TNF Alpha which has a predicted molecular weight of approximately 26 kDa.
This product is a Low Endotoxin formulation.
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Methamphetamine-induced neuroinflammation and neuronal dysfunction in the mice hippocampus: preventive effect of indomethacin.
P350 was used in immunohistochemistry to study the effect of methamphetamine in mice hippocampus
|Gonçalves J,Baptista S,Martins T,Milhazes N,Borges F,Ribeiro CF,Malva JO,Silva AP||The European journal of neuroscience (31:315)||2010|
Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice.
P350 was used in immunohistochemistry to test if IL-1beta and TNF-alpha are synthesized by overlapping or segregated populations of cells after ischemic stroke in mice
|Clausen BH,Lambertsen KL,Babcock AA,Holm TH,Dagnaes-Hansen F,Finsen B||Journal of neuroinflammation (5:null)||2008|
Tumor necrosis factor and its p55 and p75 receptors are not required for axonal lesion-induced microgliosis in mouse fascia dentata.
P350 was used in immunohistochemistry to study the relationship between tumor necrosis factor and axonal lesion-induced microgliosis in mouse fascia dentata
|Fenger C,Drojdahl N,Wirenfeldt M,Sylvest L,Jorgensen OS,Meldgaard M,Lambertsen KL,Finsen B||Glia (54:591)||2006|
Immunohistopathology of the contralateral testis of rats undergoing experimental torsion of the spermatic cord.
P350 was used in immunohistochemistry to study the immunohistopathology of the contralateral testis of rats undergoing experimental torsion of the spermatic cord
|Rodriguez MG,Rival C,Theas MS,Lustig L||Asian journal of andrology (8:576)||2006|
Involvement of tumor necrosis factor-alpha in the pathogenesis of autoimmune orchitis in rats.
P350 was used in immunohistochemistry to study the role of TNF-alpha in the pathogenesis of autoimmune orchitis in a rat model
|Suescun MO,Rival C,Theas MS,Calandra RS,Lustig L||Biology of reproduction (68:2114)||2003|
Tumour necrosis factor-alpha released by testicular macrophages induces apoptosis of germ cells in autoimmune orchitis.
P350 was used in immunocytochemistry to study the role of tumour necrosis factor alpha in autoimmune orchitis
|Theas MS,Rival C,Jarazo-Dietrich S,Jacobo P,Guazzone VA,Lustig L||Human reproduction (Oxford, England) (23:1865)||2008|
Constitutive expression of tumor necrosis factor-alpha in cytotoxic cells of teleosts and its role in regulation of cell-mediated cytotoxicity.
P350 was used in western blot to investigate the expression and the role of tumor necrosis factor alpha in cytotoxic cells of teleosts
|Praveen K,Evans DL,Jaso-Friedmann L||Molecular immunology (43:279)||2006|
The expression and functional role of nicotinic acetylcholine receptors in rat adipocytes.
P350 was used in western blot to investigate the expression and function of nicotinic acetylcholine receptor in adipocytes
|Liu RH,Mizuta M,Matsukura S||The Journal of pharmacology and experimental therapeutics (310:52)||2004|
Lower limb ischemia-reperfusion injury triggers a systemic inflammatory response and multiple organ dysfunction.
P350 was used in ELISA to investigate the effect of lower limb ischemia-reperfusion injury in immune response induction in vivo
|Yassin MM,Harkin DW,Barros D'Sa AA,Halliday MI,Rowlands BJ||World journal of surgery (26:115)||2002|
LPS-binding protein protects mice from septic shock caused by LPS or gram-negative bacteria.
P350 was used in ELISA to study the role of LPS-binding protein in sepsis
|Lamping N,Dettmer R,Schröder NW,Pfeil D,Hallatschek W,Burger R,Schumann RR||The Journal of clinical investigation (101:2065)||1998|