|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG1, kappa|
|Immunogen||Recombinant mouse TNF alpha|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Neutralization (Neu)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MM350D targets TNF Alpha in ELISA, Neu, and WB applications and shows reactivity with mouse samples.
The MM350D immunogen is recombinant mouse TNF alpha. This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
MM350D detects TNF Alpha which has a predicted molecular weight of approximately 26 kDa.
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Rapid proliferation and differentiation impairs the development of memory CD8+ T cells in early life.
MM350D was used in flow cytometry to investigate incomplete immunity against intracellular pathogens in neonates
|Smith NL,Wissink E,Wang J,Pinello JF,Davenport MP,Grimson A,Rudd BD||Journal of immunology (Baltimore, Md. : 1950) (193:177)||2014|
|Not Applicable||Not Cited||
Enhanced efficacy and reduced toxicity of multifactorial adjuvants compared with unitary adjuvants as cancer vaccines.
MM350D was used in flow cytometry to study how multifactorial adjuvants show reduced toxicity and enhanced efficacy compared to unitary adjuvants as cancer vaccines
|Ahonen CL,Wasiuk A,Fuse S,Turk MJ,Ernstoff MS,Suriawinata AA,Gorham JD,Kedl RM,Usherwood EJ,Noelle RJ||Blood (111:3116)||2008|
Dendritic-cell exosomes cross-present Toll-like receptor-ligands and activate bystander dendritic cells.
MM350D was used in blocking or activating experiment to study the ability of dendritic cell exosomes to bind TLR ligands and their role in dendritic cell sentinel function
|Sobo-Vujanovic A,Munich S,Vujanovic NL||Cellular immunology (289:119)||2014|
TNF-alpha signaling is not required for in vivo transcriptional reactivation of latent murine cytomegalovirus.
MM350D was used in blocking/activating experiment to study the role of tumor necross factor alpha in latent murine cytomegalovirus
|Zhang Z,Li Z,Yan S,Wang X,Abecassis M||Transplantation (88:640)||2009|
Intra-colonic administration of the TLR7 agonist R-848 induces an acute local and systemic inflammation in mice.
MM350D was used in blocking/activating experiment to study the inflammatory response induced by a TLR7 agonist R-848 in mice
|Karlsson A,Jägervall K,Utkovic H,Karlsson L,Rehnström E,Fredin MF,Gillberg PG,Jansson L,Michaëlsson E,Melgar S||Biochemical and biophysical research communications (367:242)||2008|
Antisense oligonucleotide blockade of tumor necrosis factor-alpha in two murine models of colitis.
MM350D was used in blocking/activating experiment to investigate the effect of antisense oligonucleotide during blockade of tumor necrosis factor alpha in two murine models of colitis
|Myers KJ,Murthy S,Flanigan A,Witchell DR,Butler M,Murray S,Siwkowski A,Goodfellow D,Madsen K,Baker B||The Journal of pharmacology and experimental therapeutics (304:411)||2003|
Lipopolysaccharide induces overexpression of MUC2 and MUC5AC in cultured biliary epithelial cells: possible key phenomenon of hepatolithiasis.
MM350D was used in blocking/activating experiment to investigate the effect of lipopolysaccharide on MUC2 and MUC5AC expression in murine-derived cultured biliary epithelial cells
|Zen Y,Harada K,Sasaki M,Tsuneyama K,Katayanagi K,Yamamoto Y,Nakanuma Y||The American journal of pathology (161:1475)||2002|
Augmented expression of tumour necrosis factor-alpha induced by lipopolysaccharide in spleen of human monocyte chemoattractant protein-1 transgenic mouse enhances the lipopolysaccharide sensitivity of the marginal zone macrophages.
MM350D was used in blocking/activating experiment to investigate the phenotype of hMCP-1 transgenic mouse stimulated by lipopolysaccharide
|Ato M,Iwabuchi K,Shimada S,Mukaida N,Onoé K||Immunology (106:554)||2002|
Murine concanavalin A-induced hepatitis is prevented by interleukin 12 (IL-12) antibody and exacerbated by exogenous IL-12 through an interferon-gamma-dependent mechanism.
MM350D was used in blocking/activating experiment to investigate the influence of interleukin 12 on ConA-induced hepatitis in mouse models
|Nicoletti F,Di Marco R,Zaccone P,Salvaggio A,Magro G,Bendtzen K,Meroni P||Hepatology (Baltimore, Md.) (32:728)||2000|
IFN-gamma, IL-12, and TNF-alpha are required to maintain reduced liver pathology in mice vaccinated with Schistosoma mansoni eggs and IL-12.
MM350D was used in blocking/activating experiment to investigate the roles of interferon gamma, interleukin 12, and tumor necrosis factor alpha in liver pathology
|Hoffmann KF,Caspar P,Cheever AW,Wynn TA||Journal of immunology (Baltimore, Md. : 1950) (161:4201)||1998|
A role for perforin in activation-induced cell death.
MM350D was used in blocking/activating experiment to investigate the function of perforin in cell death
|Spaner D,Raju K,Radvanyi L,Lin Y,Miller RG||Journal of immunology (Baltimore, Md. : 1950) (160:2655)||1998|
Modulation of TNFalpha, a determinant of acute toxicity associated with systemic delivery of first-generation and helper-dependent adenoviral vectors.
MM350D was used in ELISA to evaluate the effect of tumor necrosis factor alpha after adenoviral vector systemic administration
|Mane VP,Toietta G,McCormack WM,Conde I,Clarke C,Palmer D,Finegold MJ,Pastore L,Ng P,Lopez J,Lee B||Gene therapy (13:1272)||2006|
|Not Applicable||Not Cited||
Redirecting in vivo elicited tumor infiltrating macrophages and dendritic cells towards tumor rejection.
MM350D was used in ELISA to describe the mechanisms by which tumor-infiltrating macrophages and dendritic cells can be redirected to become potent effectors and activators of the innate and adaptive immunity
|Guiducci C,Vicari AP,Sangaletti S,Trinchieri G,Colombo MP||Cancer research (65:3437)||2005|
Temporal correlation of tumor necrosis factor-alpha release, upregulation of pulmonary ICAM-1 and VCAM-1, neutrophil sequestration, and lung injury in diet-induced pancreatitis.
MM350D was used in ELISA to investigate the interplay of tumor necrosis factor-alpha, ICAM-1 and VCAM-1 in diet-induced pancreatitis
|Lundberg AH,Granger N,Russell J,Callicutt S,Gaber LW,Kotb M,Sabek O,Gaber AO||Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract (4:248)||2000|
Resistance of Staphylococcal enterotoxin B- induced proliferation and apoptosis to the effects of dexamethasone in mouse lymphocyte cultures.
MM350D was used in ELISA to investigate the role of dexamethasone in mouse lymphocyte cultures
|Weng CF,Zhao W,Fegeding KV,Komisar JL,Tseng J||International immunology (11:787)||1999|