|Tested species reactivity||Pig|
|Published species reactivity||Pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant porcine TNF alpha (Baculovirus-derived)|
|Tested Applications||Dilution *|
|ELISA (ELISA)||3 µg/mL (coating)|
|Western Blot (WB)||0.02 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 3 publications below|
MP391 detects natural and recombinant TNF alpha, with a predicted molecular weight of approximately 25 kDa.
This is a carrier-free low endotoxin formulation, <1.0 EU/mg.
This product can be used as a coating antibody in a sandwich ELISA (100 µg/well) in combination with biotin-labeled TNFa monoclonal antibody (Product # M302B). For Western blot, this antibody has been successfully used in conjunction with 0.02 µg/mL Biotin-conjugated Goat-anti-Mouse IgG (Product # 31800), 0.05 µg/mL HRP-conjugated Streptavidin (Product # N100), and recombinant Porcine TNFa (Product # RPTNFAI), followed by visualization using SuperSignal® West Pico Chemiluminescent Substrate (Product # 34080).
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Classical swine fever virus induces tumor necrosis factor-alpha and lymphocyte apoptosis.
MP391 was used in immunohistochemistry to study the effect of classical swine fever virus on tumor necrosis factor alpha production and lymphocyte apoptosis
|Choi C,Hwang KK,Chae C||Archives of virology (149:875)||2004|
Percutaneous plasma decompression alters cytokine expression in injured porcine intervertebral discs.
MP391 was used in immunohistochemistry to study the effect of percutaneous plasma decompression on degenerating porcine intervertebral discs
|O'Neill CW,Liu JJ,Leibenberg E,Hu SS,Deviren V,Tay BK,Chin CT,Lotz JC||The spine journal : official journal of the North American Spine Society (4:88)||2004|
Lipopolysaccharide induces inflammatory cytokines in the pig amnion.
MP391 was used in immunohistochemistry to investigate the effect of lipopolysaccharide on cytokine production in the pig amnion
|Trebichavský I,Splíchal I,Zahradnícková M,Splíchalová A,Mori Y||Veterinary immunology and immunopathology (87:11)||2002|