|Tested species reactivity||Pig|
|Published species reactivity||Pig, Rat, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant porcine TNF alpha (Baculovirus-derived)|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-0.25 µg/mL (coating)|
|Western Blot (WB)||0.02 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MP390 detects natural and recombinant TNF alpha, with a predicted molecular weight of approximately 25 kDa. This antibody will not cross-react with human or mouse TNFa, mouse TNFb, or porcine TNFb.
This is a carrier-free low endotoxin formulation, <1.0 EU/mg.
This product can be used as a coating antibody in a sandwich ELISA (100 µg/well) in combination with biotin-labeled TNFa monoclonal antibody (Product # M302B, 0.1-0.65 µg/mL). For Western blot, this antibody has been successfully used in conjunction with 0.02 µg/mL Biotin-conjugated Goat-anti-Mouse IgG (Product # 31800), 0.05 µg/mL HRP-conjugated Streptavidin (Product # N100), and recombinant Porcine TNFa (Product # RPTNFAI), followed by visualization using SuperSignal® West Pico Chemiluminescent Substrate (Product # 34080).
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Development of a cytometric bead array screening tool for the simultaneous detection of pro-inflammatory cytokines in porcine plasma.
MP390 was used in ELISA to develop a cytometric bead array to detect porcine cytokines and acute phase proteins
|Wyns H,Croubels S,Demeyere K,Watteyn A,De Backer P,Meyer E||Veterinary immunology and immunopathology (151:28)||2013|
Detection of monokines in paraffin-embedded tissues of pigs using polyclonal antibodies.
MP390 was used in ELISA to investigate the reliability of antibodies for the measurement of monokines in paraffin-embedded pig tissues
|Salguero FJ,Mekonnen T,Ruiz-Villamor E,Sánchez-Cordón PJ,Gómez-Villamandos JC||Veterinary research (32:601)||2002|
Lipopolysaccharide-induced impairment of classical swine fever virus infection in monocytic cells is sensitive to 2-aminopurine.
MP390 was used in ELISA to study the effect of lipopolysaccharide and 2-aminopurine on classical swine fever virus infection
|Knoetig SM,McCullough KC,Summerfield A||Antiviral research (53:75)||2002|
Does nanocrystalline silver have a transferable effect?
MP390 was used in immunohistochemistry to study the antiinflammatory effect of nanocrystalline silver
|Nadworny PL,Landry BK,Wang J,Tredget EE,Burrell RE||Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society (18:254)||2010|
Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis.
MP390 was used in immunohistochemistry to evaulate the benefit of nanocrystalline silver in porcine contact dermatitis
|Nadworny PL,Wang J,Tredget EE,Burrell RE||Journal of inflammation (London, England) (7:null)||2010|
Naringenin inhibits neointimal hyperplasia following arterial reconstruction with interpositional vein graft.
MP390 was used in immunohistochemistry to study the role of Naringenin in development of neointima following arterial reconstruction
|Cayci C,Wahlquist TC,Seckin SI,Ozcan V,Tekinay AB,Martens TP,Oz MC,Ascherman JA||Annals of plastic surgery (64:105)||2010|
Production of tumor necrosis factor-alpha from porcine nucleus pulposus cells at various time points in cell culture under conditions of nutritional deficiency.
MP390 was used in immunohistochemistry to investigate the changes of tumor necrosis factor alpha expression in pigs under starvation conditions
|Murata Y,Olmarker K,Larsson K,Takahashi K,Rydevik B||Cytokine (34:206)||2006|
|Not Applicable||Not Cited||
Effects of porcine reproductive and respiratory syndrome virus-infected antigen-presenting cells on T cell activation and antiviral cytokine production.
MP390 was used in flow cytometry to show that porcine reproductive and respiratory syndrome virus can suppress T cells
|Charerntantanakul W,Platt R,Roth JA||Viral immunology (19:646)||2007|
Cellular changes and cytokine expression in the ilea of gnotobiotic piglets resulting from peroral Salmonella typhimurium challenge.
MP390 was used in immunocytochemistry to investigate the changes of cytokine gene expression in piglets infected with Salmonella typhimurium
|Trebichavský I,Dlabac V,Reháková Z,Zahradnícková M,Splíchal I||Infection and immunity (65:5244)||1997|