|Tested species reactivity||Horse|
|Published species reactivity||Bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant Equine TNF alpha|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 1 publications below|
PETNFAI targets TNF Alpha in ELISA, and WB applications and shows reactivity with Equine samples.
The PETNFAI immunogen is recombinant Equine TNF alpha.
PETNFAI detects TNF Alpha which has a predicted molecular weight of approximately 25 kDa.
The PETNFAI equine TNFa polyclonal antibody has successfully been paired as the coating antibody in a sandwich ELISA with biotinylated detection antibody PETNFABI. Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cytokine production and proliferation upon in vitro oligodeoxyribonucleotide stimulation of equine peripheral blood mononuclear cells.
PETNFAI was used in ELISA to study cytokine production and proliferation by equine peripheral blood mononuclear cells stimulated with oligonucleotides
|Wattrang E,Palm AK,Wagner B||Veterinary immunology and immunopathology (146:113)||2012|