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|Tested species reactivity||Eubacteria|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Diphtheria toxoid (formaldehyde inactivated Diphtheria toxin).|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
HYB 123-09-02 detects Corynebacterium diphtheriae toxoid in bacteria samples.
HYB 123-09-02 has been successfully used in ELISA and Western blot procedures. Where a band (~63kDa) is detected corresponding to the toxin proenzyme.
HYB 123-09-02 immunogen is Diphtheria toxoid prepared from C.diphtheria subsp. Intermedius Park-Williams no. 8 by formaldehyde inactivation.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/ml
Diphtheria toxin (DT) is secreted by certain strains of Corynebacterium diphtheriae and catalyzes the ADP-ribosylation of eukaryotic aminoacyl-transferase II (EF-2) using NAD as a substrate. This reaction forms the basis for its toxicity toward eukaryotic organisms. Diphtheria toxin is synthesized and excreted as a proenzyme, composed of a single polypeptide chain having a molecular weight of approximately 63 kDa. Two covalent alterations in structure are necessary for expression of its enzymatic activity. First, mild proteolysis results in the formation of "nicked toxin," which is enzymatically inactive and consists of two major fragments, A and B, linked by a disulfide bond. Reduction of the nicked toxin with thiols releases the N-terminal A fragment (~24 kDa) which is enzymatically active. The C-terminal B fragment (~39 kDa) has no apparent enzymatic activity, but is required for toxicity. Evidence suggests that the B fragment is responsible for recognizing and binding the toxin to cell surface receptors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
C. diphtheriae; Diphtheria