|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Synthetic peptide corresponding to residues R(196) G Q S C N N Q P L N H K V Y M R N S K Y P E D L(220) of mouse Fas Ligand.|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunocytochemistry (ICC)||10 µg/ml|
|Immunofluorescence (IF)||10 µg/ml|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-25850 has been successfully used in immunocytochemistry, FACS and Western blot procedures.
The MA1-25850 immunogen is a synthetic peptide corresponding to residues R(196) G Q S C N N Q P L N H K V Y M R N S K Y P E D L(220) of mouse Fas Ligand.
Fas / APO1 (CD95) is an important member of the tumour necrosis factor (TNF) superfamily involved in membrane-mediated apoptosis. Ligation of Fas by Fas Ligand or an anti-Fas cross-linking antibody, triggers activation of the caspase cascade. Functional impairment of the Fas / Fas Ligand system is associated with the development and progression of malignancies. Fas gene mutations have been suggested to have a role in testicular germ cell tumours. Tumour cells frequently exhibit de novo expression of Fas Ligand, which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells. The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/Fas Ligand system. Fas is believed to be involved in various autoimmune diseases including ulcerative colitis, Graves disease, and rheumatoid arthritis. Fas expression on gastric epithelial cells in patients infected with H. Pylori is responsible for the accelerated apoptosis of the cells. Serum Fas Ligand concentration has also been shown to be associated with atherosclerosis and inflammatory disease, in patients with hypertension.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.