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Neutralization assay of recombinant human Lymphotoxin-a/TNF-ß induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by recombinant human Lymphotoxin-a/TNF-ß (0.25 ng/mL) is neutralized (green line) by increasing concentrations of mouse Anti-human Lymphotoxin-a/TNF-ß Monoclonal Antibody (Product # MA5-23801). The ND50 is typically 0.03-0.09 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||E. coli-derived recombinant human Lymphotoxin-alpha/TNF-beta|
|Storage buffer||PBS with 5% trehalose|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-0.4 µg/ml (Detection), 2-8 µg/ml (Capture)|
|Neutralization (Neu)||0.03-0.09 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In sandwich ELISAs, approximately 10% cross-reactivity with recombinant mouse TNF- beta is observed and no cross-reactivity with bovine FGF acidic, recombinant human (rh) GM-CSF, rhIL-1 alpha, rhIL-1 beta, rhIL-2, rhIL-3, rhIL-4, rhIL-6, rhIL-8, rhTNF- alpha, rhPDGF, rpPDGF, rhTGF- beta 1, recombinant porcine (rp) TGF- beta 1, rpTGF- beta 1.2, or rpTGF- beta 2 is observed.
Reconstitute at 0.5 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
The encoded protein, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4 and psoriatic arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
LT-alpha; LTA; TNF superfamily, member 1; TNF-beta; TNFB; TNFSF1; tumor necrosis factor beta; tumor necrosis factor ligand 1E; tumor necrosis factor ligand superfamily member 1
LT; LTA; TNFB; TNFSF1; TNLG1E