Western blot analysis of Hemaggutinin using recombinant seasonal Hemagglutinin (lanes A and B) and swine-origin Hemagglutinin (lanes C and D) with a Seasonal H1N1 Hemagglutinin polyclonal antibody (Product # PA5-20712) at 2 ug/mL (lanes A and C) and a Swine H1N1 Hemagglutinin polyclonal antibody (Product # PA5-20731) at 2 ug/mL (lanes B and D).
|Tested species reactivity||Virus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide from the Hemagglutinin protein.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects a peptide sequence that differs from Product # PA5-20713 and PA5-20715, and can be used as a cognate pair with Product # PA5-20731.
PA5-20712 can be used with blocking peptide PEP-0827.
Influenza A virus is a major public health threat, killing more than 30, 000 people per year in the USA. In early 2009, a novel swine-origin influenza A (H1N1) virus was identified in specimens obtained from patients in Mexico and the United States. The virus spread quickly around the world and on June 11, 2009, the World Health Organization declared it a pandemic. Influenza A virus has one of sixteen possible Hemagglutinin (HA) surface proteins and one of nine possible Neuraminidase (NA) surface proteins. The Hemagglutinin protein facilitates viral attachment while Neuraminidase is involved in viral release. These proteins also elicit immune responses that prevent infection or independently reduce viral replication. The genetic make-up of this swine flu virus is unlike any other: it is an H1N1 strain that combines a triple assortment first identified in 1998 including human, swine, and avian influenza with two new pig H3N2 virus genes from Eurasia, themselves of recent human origin. The distinct antigenic properties of the new swine virus compared with seasonal influenza A (H1N1) virus suggest that human immunity against new swine influenza virus is limited, although the age distribution of reported cases suggests some degree of protection in older age groups.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.