|Immunocytochemistry (ICC)||10 ug/ml|
|Immunofluorescence (IF)||10 ug/ml|
|Immunohistochemistry (IHC)||2 µg/ml|
|Western Blot (WB)||1 µg/ml|
|Tested Species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids near the amino terminus of mouse ICAD.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
In Western blot applications, this antibody detects a band at ~45kDa. A suggested positive control is mouse lung tissue lysate.
PA5-19889 can be used with blocking peptide PEP-0017.
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which is cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF was identified as a DNase inhibitor designated ICAD, for inhibitor of caspase-activated DNase. Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DFF-45; DNA fragmentation factor 45 kDa subunit; DNA fragmentation factor subunit alpha; ICAD; Inhibitor of CAD; RP23-121D17.3, A330085O09Rik, DFF35, Dff45, ICAD, ICAD-L, ICAD-S
Gene Aliases: A330085O09Rik; DFF35; Dff45; Dffa; ICAD; ICAD-L; ICAD-S
UniProt ID: (Mouse) O54786
Entrez Gene ID: (Mouse) 13347
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