|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2a|
|Conjugate||Alexa Fluor® 647|
|Storage buffer||PBS with 4mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Extension of the germinal center stage of B cell development promotes autoantibodies in BXD2 mice.
MCD0421 was used in immunohistochemistry - frozen section to determine the mechanisms by which RGS13 promotes the generation of pathogenic autoantibodies in germinal centers.
|Wang JH,New JS,Xie S,Yang P,Wu Q,Li J,Luo B,Ding Y,Druey KM,Hsu HC,Mountz JD||Arthritis and rheumatism (65:2703)||2013|
|Not Applicable||Not Cited||
Marginal zone precursor B cells as cellular agents for type I IFN-promoted antigen transport in autoimmunity.
MCD0421 was used in immunohistochemistry to study type I IFN and its role in regulating the migration response of antigen delivery by B cells into lymphoid follicles in autoimmune mice
|Wang JH,Li J,Wu Q,Yang P,Pawar RD,Xie S,Timares L,Raman C,Chaplin DD,Lu L,Mountz JD,Hsu HC||Journal of immunology (Baltimore, Md. : 1950) (184:442)||2010|