|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2a|
|Storage buffer||PBS with 4mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mice with an absent stress response are protected against ischemic renal injury.
MCD0428 was used in flow cytometry to determine if HSPs ameliorate ischemic renal injury in vivo using HSF-1 KO mice
|Sreedharan R,Chen S,Miller M,Haribhai D,Williams CB,Van Why SK||Kidney international (86:515)||2014|
The expression of Sox17 identifies and regulates haemogenic endothelium.
MCD0428 was used in flow cytometry to identify Sox7 as a key regulator of haemogenic endothelial development.
|Clarke RL,Yzaguirre AD,Yashiro-Ohtani Y,Bondue A,Blanpain C,Pear WS,Speck NA,Keller G||Nature cell biology (15:502)||2013|
|Not Applicable||Not Cited||
Notch3 is dispensable for thymocyte ß-selection and Notch1-induced T cell leukemogenesis.
MCD0428 was used in flow cytometry to determine the importance of ligand-activated N3 in promoting beta-selection and N1 intracellular domain-induced T cell leukemogenesis
|Suliman S,Tan J,Xu K,Kousis PC,Kowalski PE,Chang G,Egan SE,Guidos C||PloS one (6:null)||2011|
Identification of T-cell epitopes in Francisella tularensis using an ordered protein array of serological targets.
MCD0428 was used in flow cytometry to identify T-cell epitopes of Francisella tularensis.
|Valentino MD,Maben ZJ,Hensley LL,Woolard MD,Kawula TH,Frelinger JA,Frelinger JG||Immunology (132:348)||2011|
Therapeutic memory T cells require costimulation for effective clearance of a persistent viral infection.
MCD0428 was used in flow cytometry to evaluate immunotherapeutic regimens in mice chronically infected with lymphocytic choriomeningitis virus
|Garidou L,Heydari S,Truong P,Brooks DG,McGavern DB||Journal of virology (83:8905)||2009|
Chronic follicular bronchiolitis requires antigen-specific regulatory T cell control to prevent fatal disease progression.
MCD0428 was used in flow cytometry to examine Treg cell control of chronic autoimmunity in a lymphoreplete host.
|Schmitt EG,Haribhai D,Jeschke JC,Co DO,Ziegelbauer J,Yan K,Iwakura Y,Mishra MK,Simpson P,Salzman NH,Williams CB||Journal of immunology (Baltimore, Md. : 1950) (191:5460)||2013|
Depletion of B2 but not B1a B cells in BAFF receptor-deficient ApoE mice attenuates atherosclerosis by potently ameliorating arterial inflammation.
MCD0428 was used in flow cytometry to study the development of atherosclerosis in BAFF-R deficient ApoE(-/-) mice.
|Kyaw T,Tay C,Hosseini H,Kanellakis P,Gadowski T,MacKay F,Tipping P,Bobik A,Toh BH||PloS one (7:null)||2012|
Granzyme B regulates antiviral CD8+ T cell responses.
MCD0428 was used in flow cytometry to determine the role of granzyme B in immune regulation during viral infections.
|Salti SM,Hammelev EM,Grewal JL,Reddy ST,Zemple SJ,Grossman WJ,Grayson MH,Verbsky JW||Journal of immunology (Baltimore, Md. : 1950) (187:6301)||2011|