Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Rabbit anti eIF2alpha [pS52] phosphospecific polyclonal antibody. Left: Image shows immunocytochemical analysis of eIF2alpha [pS52] in rat brain (mediodorsal neocortex) sections following traumatic axonal injury (TAI) (24 h). Control (prior to injury induction) shows low basal levels of eIF2alpha [pS52] (see inset). Right: Image shows double labeling of APP (red) and eIF2alpha [pS52] (green) in neurons subjected to traumatic axonal injury (TAI). Inset (above): higher magnification of neuronal soma with TAI. eIF2alpha [pS52] immunofluorescence is localized in cytoplasm of neuronal cell body. (Singleton, R.H., et al. (2002) J. Neurosci. 22(3):791-802.)
|Tested species reactivity||Human, Mouse, Yeast|
|Published species reactivity||Yeast, Rat, Fruit fly, Virus, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human eIF2a that contains serine 52. This region is conserved among many species including rat, pig, cow, fruit fly and yeast.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The cytotoxic effect of the NOS-mediated oxidative stress in MCF-7 cells after PbCl2 exposure.
44-728G was used in western blot to examine the NOS signaling pathway during Pb-induced cytotoxicity.
|Zhong L,Wang L,Xu L,Liu Q,Jiang L,Zhi Y,Lu W,Zhou P||Environmental toxicology (31:601)||2016|
Hepatocyte Nicotinamide Adenine Dinucleotide Phosphate Reduced Oxidase 4 Regulates Stress Signaling, Fibrosis, and Insulin Sensitivity During Development of Steatohepatitis in Mice.
44-728G was used in western blot to investigate the effects of nicotinamide adenine dinucleotide phosphate reduced oxidase 4 in liver tissues from patients with NASH and mice with steatohepatitis.
|Bettaieb A,Jiang JX,Sasaki Y,Chao TI,Kiss Z,Chen X,Tian J,Katsuyama M,Yabe-Nishimura C,Xi Y,Szyndralewiez C,Schröder K,Shah A,Brandes RP,Haj FG,Török NJ||Gastroenterology (149:468)||2015|
Impaired protein translation in Drosophila models for Charcot-Marie-Tooth neuropathy caused by mutant tRNA synthetases.
44-728G was used in western blot to develop a Drosophila model for Charcot-Marie-Tooth by mutating glycyl-tRNA synthetase
|Niehues S,Bussmann J,Steffes G,Erdmann I,Köhrer C,Sun L,Wagner M,Schäfer K,Wang G,Koerdt SN,Stum M,Jaiswal S,RajBhandary UL,Thomas U,Aberle H,Burgess RW,Yang XL,Dieterich D,Storkebaum E||Nature communications (6:null)||2015|
Stress-resistant Translation of Cathepsin L mRNA in Breast Cancer Progression.
44-728G was used in western blot to study how cysteine protease cathepsin L expression contributes to different types of cancer.
|Tholen M,Wolanski J,Stolze B,Chiabudini M,Gajda M,Bronsert P,Stickeler E,Rospert S,Reinheckel T||The Journal of biological chemistry (290:15758)||2015|
Preventing proteostasis diseases by selective inhibition of a phosphatase regulatory subunit.
44-728G was used in western blot to show that Sephin1, a selective inhibitor of protein phosphatase 1, protects mice from protein-misfolding disease.
|Das I,Krzyzosiak A,Schneider K,Wrabetz L,D'Antonio M,Barry N,Sigurdardottir A,Bertolotti A||Science (New York, N.Y.) (348:239)||2015|
The small molecule ISRIB reverses the effects of eIF2α phosphorylation on translation and stress granule assembly.
44-728G was used in western blot to study mRNA transcript production during the integrated stress response.
|Sidrauski C,McGeachy AM,Ingolia NT,Walter P||eLife (4:null)||2015|
Identification of protein disulfide isomerase 1 as a key isomerase for disulfide bond formation in apolipoprotein B100.
44-728G was used in western blot to elucidate both MTP-dependent and -independent roles of PDI1.
|Wang S,Park S,Kodali VK,Han J,Yip T,Chen Z,Davidson NO,Kaufman RJ||Molecular biology of the cell (26:594)||2015|
The role of NOS-mediated ROS accumulation in an early phase Cu-induced acute cytotoxicity in MCF-7 cells.
44-728G was used in western blot to test if the nitric oxide synthase signaling pathway contributes to Cu-induced acute cytotoxicity
|Zhong L,Wang L,Xu L,Liu Q,Jiang L,Zhi Y,Lu W,Zhou P||Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine (28:113)||2015|
The role of nitric oxide synthase signaling pathway in the Zn-induced cellular responses in MCF-7 cells.
44-728G was used in western blot to test if zinc regulates NOS signaling
|Zhong L,Wang L,Xu L,Liu Q,Jiang L,Zhi Y,Lu W,Zhou P||Environmental toxicology and pharmacology (38:783)||2014|
Mmu-miR-615-3p regulates lipoapoptosis by inhibiting C/EBP homologous protein.
44-728G was used in western blot to identify specific microRNAs regulating C/EBP homologous protein expression during palmitate-induced ER stress
|Miyamoto Y,Mauer AS,Kumar S,Mott JL,Malhi H||PloS one (9:null)||2014|
The role of constitutive nitric-oxide synthase in ultraviolet B light-induced nuclear factor κB activity.
44-728G was used in western blot to study NF-kappaB in UV-irradiated cells
|Tong L,Wu S||The Journal of biological chemistry (289:26658)||2014|
An AUG codon conserved for protein function rather than translational initiation: the story of the protein sElk1.
44-728G was used in western blot to study sElk1 protein
|Legrand N,Araud T,Conne B,Kuijpers O,Jaquier-Gubler P,Curran J||PloS one (9:null)||2014|
Endoplasmic reticulum stress impairs IL-4/IL-13 signaling through C/EBPβ-mediated transcriptional suppression.
44-728G was used in western blot to report that ER stress leads to coordinated transcriptional suppression of cytokine signaling.
|Arensdorf AM,Rutkowski DT||Journal of cell science (126:4026)||2013|
Receptor for activated C-kinase (RACK1) homolog Cpc2 facilitates the general amino acid control response through Gcn2 kinase in fission yeast.
44-728G was used in western blot to examine the regulatory function of RACK on Gcn2-mediated general amino acid control response.
|Tarumoto Y,Kanoh J,Ishikawa F||The Journal of biological chemistry (288:19260)||2013|
PKR-like endoplasmic reticulum kinase is necessary for lipogenic activation during HCMV infection.
44-728G was used in western blot to study the induction of PERK expression during human cytomegalovirus infection and its role in activating lipogenesis.
|Yu Y,Pierciey FJ,Maguire TG,Alwine JC||PLoS pathogens (9:null)||2013|
An upstream open reading frame modulates ebola virus polymerase translation and virus replication.
44-728G was used in western blot to characterize the Zaire ebolavirus 5'-UTRs.
|Shabman RS,Hoenen T,Groseth A,Jabado O,Binning JM,Amarasinghe GK,Feldmann H,Basler CF||PLoS pathogens (9:null)||2013|
Herpes simplex virus-1 disarms the unfolded protein response in the early stages of infection.
44-728G was used in western blot to assess the effects of HSV- on the unfolded protein response during lytic replication.
|Burnett HF,Audas TE,Liang G,Lu RR||Cell stress & chaperones (17:473)||2012|
|Yeast||Not Cited||Induction of a G1-S checkpoint in fission yeast.||Bøe CA,Krohn M,Rødland GE,Capiaghi C,Maillard O,Thoma F,Boye E,Grallert B||Proceedings of the National Academy of Sciences of the United States of America (109:9911)||2012|
|Mouse||Not Cited||Murine cytomegalovirus targets transcription factor ATF4 to exploit the unfolded-protein response.||Qian Z,Xuan B,Chapa TJ,Gualberto N,Yu D||Journal of virology (86:6712)||2012|
|Virus||Not Cited||Accumulation of autophagosomes in Semliki Forest virus-infected cells is dependent on expression of the viral glycoproteins.||Eng KE,Panas MD,Murphy D,Karlsson Hedestam GB,McInerney GM||Journal of virology (86:5674)||2012|
Skeletal muscle amino acid transporter expression is increased in young and older adults following resistance exercise.
44-728G was used in western blot to study the role of amino acid transporters in muscle protein anabolism.
|Drummond MJ,Fry CS,Glynn EL,Timmerman KL,Dickinson JM,Walker DK,Gundermann DM,Volpi E,Rasmussen BB||Journal of applied physiology (Bethesda, Md. : 1985) (111:135)||2011|
|Rat||1:3000||Ectopic expression of eIF2Bepsilon in rat skeletal muscle rescues the sepsis-induced reduction in guanine nucleotide exchange activity and protein synthesis.||Tuckow AP,Vary TC,Kimball SR,Jefferson LS||American journal of physiology. Endocrinology and metabolism (299:E241)||2010|
|Human||Not Cited||A crucial role for RACK1 in the regulation of glucose-stimulated IRE1alpha activation in pancreatic beta cells.||Qiu Y,Mao T,Zhang Y,Shao M,You J,Ding Q,Chen Y,Wu D,Xie D,Lin X,Gao X,Kaufman RJ,Li W,Liu Y||Science signaling (3:null)||2010|
|Rat||Not Cited||Impaired dexamethasone-mediated induction of tryptophan 2,3-dioxygenase in heme-deficient rat hepatocytes: translational control by a hepatic eIF2alpha kinase, the heme-regulated inhibitor.||Liao M,Pabarcus MK,Wang Y,Hefner C,Maltby DA,Medzihradszky KF,Salas-Castillo SP,Yan J,Maher JJ,Correia MA||The Journal of pharmacology and experimental therapeutics (323:979)||2007|
|Mouse||Not Cited||The double-stranded RNA-activated protein kinase mediates radiation resistance in mouse embryo fibroblasts through nuclear factor kappaB and Akt activation.||von Holzen U,Pataer A,Raju U,Bocangel D,Vorburger SA,Liu Y,Lu X,Roth JA,Aggarwal BB,Barber GN,Keyomarsi K,Hunt KK,Swisher SG||Clinical cancer research : an official journal of the American Association for Cancer Research (13:6032)||2007|
|Yeast||Not Cited||Mechanistic link between PKR dimerization, autophosphorylation, and eIF2alpha substrate recognition.||Dey M,Cao C,Dar AC,Tamura T,Ozato K,Sicheri F,Dever TE||Cell (122:901)||2005|
|Growth arrest and DNA damage-inducible protein GADD34 targets protein phosphatase 1 alpha to the endoplasmic reticulum and promotes dephosphorylation of the alpha subunit of eukaryotic translation initiation factor 2.||Brush MH,Weiser DC,Shenolikar S||Molecular and cellular biology (23:1292)||2003|
|Mouse||Not Cited||The PERK eukaryotic initiation factor 2 alpha kinase is required for the development of the skeletal system, postnatal growth, and the function and viability of the pancreas.||Zhang P,McGrath B,Li S,Frank A,Zambito F,Reinert J,Gannon M,Ma K,McNaughton K,Cavener DR||Molecular and cellular biology (22:3864)||2002|
Phosphorylation of eIF2 facilitates ribosomal bypass of an inhibitory upstream ORF to enhance CHOP translation.
44-728G was used in immunocytochemistry to investigate the relationship between eIF2 phosphorylation and CHOP during the stress response.
|Palam LR,Baird TD,Wek RC||The Journal of biological chemistry (286:10939)||2011|
EIF-2; eIF-2-alpha; eIF-2A; EIF-2alpha; EIF2; EIF2A; EIF2S1; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; eukaryotic translation initiation factor 2A
0910001O23Rik; 2410026C18Rik; 35kDa; EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A; eIF2alpha; EIF2S1