|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide corresponding to 17 amino acids form near the carboxy terminus of human NAK/TBK1.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||10 µg/ml|
|Western Blot (WB)||0.5-1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In Western blot applications, this antibody detects a band at ~84kDa. A suggested positive control is MOLT4 cell lysate.
PA5-19968 can be used with blocking peptide PEP-0093.
Nuclear factor kappa B (NF-kappa-B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappa-B mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kappa-B is associated with Ikappa-B proteins in the cell cytoplasm, which inhibit NF-kappa-B activity. Phosphorylation of I-kappaB by Ikappa-B kinase (IKK) complex leads to degradation of I-kappaB and activation of NF-kappa-B. The IKK complex contains IKK-a, IKK-b, and IKK-gamma. A novel IKK related kinase was recently identified and designated TBK1 (TANK-binding kinase 1), NAK (NF-kappa-B-activating kinase), and T2K. NAK/TBK1 activates IKK-b through direct phosphorylation. NAK/TBK1 is activated by growth factors and PMA and mediates IKK and NF-kappa-B activation in response to growth factors. NAK/TBK1 functions upstream of NIK and the IKK complex. NAK/TBK1 is also critical in protecting embryonic liver from apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.