|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgM|
|Immunogen||Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.|
|Purification||selective precipitation, Size-exclusion chromatography|
|Storage buffer||TBS, pH 8.0|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
|Western Blot (WB)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For ICC staining, membrane permeabilization with acetone is essential.
Purity is >95% by SDS-PAGE.
VCP (valosin-containing protein), is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm, this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.