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Immunocytochemical analysis of DPPA2 was detected in immersion fixed BG01V human embryonic stem cells using Sheep Anti-human DPPA2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47898) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper pane and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei.
|Tested species reactivity||Human|
|Host / Isotype||Sheep / IgG|
|Immunogen||E. coli-derived recombinant human DPPA2 Ser2-Thr174|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 5% trehalose|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||5-15 µg/ml|
|Immunofluorescence (IF)||5-15 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
cancer/testis antigen 100, developmental pluripotency-associated protein 2, embryonic stem cell (ESC) associated transcript 15-2, pluripotent embryonic stem cell-related gene 1 protein, CT100, ECSA, PESCRG1, developmental pluripotency associated 2, PESCRG1cancer/testis antigen 100
PESCRG1, CT100, ECAT15-2