Western blot analysis of FOXO1 (S256) in recombinant fusion protein containing 1) a phosphorylated serine at position 256 and 2) an unphosphorylated serine at position 256, using a Phospho-FOXO1A pSer256 polyclonal antibody (Product # PA5-23328) at 0.1 ug/ml.
|Tested species reactivity||Bovine, Dog, Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (251 RRRAA(S)MDNNSKFAK 265) of Phospho-FOXO1A pSer256 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:100-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
FOXO1 is a protein belonging to the forkhead family of transcription activators which is characterized by a distinct forkhead domain. The specific function of this protein has not yet been determined, however, it may play a role in myogenic growth and differentiation. It is also known to have a tumor suppressor function by inhibiting cell proliferation and survival. When fused to PAX3 it is known to cause alveolar rhabdomyosarcoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.