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Immunohistochemical analysis of Kallikrein 11 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-human Kallikrein 11 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47164) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB (brown and counterstained with hematoxylin (blue).
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Mouse myeloma cell line NS0-derived recombinant human Kallikrein 11 Ile54-Asn282|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 5% trehalose|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5-15 µg/ml|
|Immunoprecipitation (IP)||25 µg/ml|
|Western Blot (WB)||0.1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) Kallikrein 1, rhKallikrein 3, rhKallikrein 5, and rhKallikrein 9 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Kallikreins (KLKs) belong to the serine protease family of proteolytic enzymes. Human pancreatic/renal KLK encodes for the KLK1 enzyme, which is involved in post-translational processing of polypeptide precursors. The function of the other members of KLK gene family is currently unknown, but evidence suggests that many KLKs are implicated in carcinogenesis. The human KLK gene family consists of 15 serine proteases. The human KLK genes are clustered on chromosome 19q13. Unlike other kalllikreins, the KLK4-15 encoded proteases are less related and do not contain a conventional KLK loop. Clusters of genes exhibit high prostatic (KLK2-4, KLK15) or pancreatic (KLK6-13) expression . KLK2 is also known as glandular kallikrein 2, tissue kallikrein or HGK-1, and KLK3 is known as prostate-specific antigen (PSA). Both KLK2 and KLK3 have important applications in prostate cancer and breast cancer diagnostics. KLK4, KLK5, KLK9, KLK13, KLK12 and KLK14 have been previously known as KLK-L1, KLK-L2, KLK-L3, KLK-L4, KLK-L5 and KLK-L6, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
hippostasin; hK11; Kallikrein 11; Kallikrein-11 inactive chain 1; Kallikrein-11 inactive chain 2; kallikrein-related peptidase 11; Kallikrein11; KLK11; PRSS20; serine protease 20; TLSP; trypsin-like protease; UNQ649/PRO1279
KLK11; PRSS20; TLSP; UNQ649/PRO1279