Human peripheral blood from normal donors was stained using a combination of anti-human CD3 monoclonal antibody (clone: S4.1), for the cell surface antigens, and anti-human / mouse Zap-70 monoclonal antibody (clone: 1E7.2), for intracellular detection. The cells were fixed and / or permeabilized using Fix and Perm solution (cat.# GAS-003 / 004). The profiles represent lymphocyte gate.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||GST-fusion protein of Human ZAP70, amino acids 282-307|
|Conjugate||Alexa Fluor® 488|
|Storage buffer||PBS with 4mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Based on our testing, publications, and results reported from customers, the Alexa Fluor® 488 dye provides the best fluorescein (FITC) substitute.
Mutations in ZAP-70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP-70 expression also defines a subset of Chronic Lymphocytic Leukaemia (CLL) in patients with unmutated Ig gene and poor clinical course.
Analyte Specific Reagent
Sorafenib induces cell death in chronic lymphocytic leukemia by translational downregulation of Mcl-1.
MHZAP7020 was used in flow cytometry to elucidate the mechanisms of sorafenib-induced cell death.
|Huber S,Oelsner M,Decker T,zum Büschenfelde CM,Wagner M,Lutzny G,Kuhnt T,Schmidt B,Oostendorp RA,Peschel C,Ringshausen I||Leukemia (25:838)||2011|
High lymphoid enhancer-binding factor-1 expression is associated with disease progression and poor prognosis in chronic lymphocytic leukemia.
MHZAP7020 was used in flow cytometry to report that LEF1 expression is associated with poor prognosis and disease progression of chronic lymphocytic leukemia.
|Erdfelder F,Hertweck M,Filipovich A,Uhrmacher S,Kreuzer KA||Hematology reports (2:null)||2010|