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|Tested species reactivity||Human , Mouse , Primate , Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of human ACTA2 expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1/200 - 1/400|
|Immunofluorescence (IF)||1/200 - 1/1000|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
MA5-15805 targets ACTA2 in FACS, IF, and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples.
The MA5-15805 immunogen is purified recombinant fragment of human ACTA2 expressed in E. Coli.
MA5-15805 detects ACTA2 which has a predicted molecular weight of approximately 42kDa.
Actin,alpha 2, smooth muscle, aorta, major constituent of thin filaments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Regulation of fibroblast lipid storage and myofibroblast phenotypes during alveolar septation in mice.
MA5-15805 was used in flow cytometry to examine pdgfralpha gene expression in mesenchymal progenitors and differentiation in lung fibroblasts
|McGowan SE,McCoy DM||American journal of physiology. Lung cellular and molecular physiology (307:L618)||2014|
actin, alpha, vascular smooth muscle; actin, aortic smooth muscle; alpha 2 actin; alpha-actin-2; alpha-cardiac actin; alpha-sma; cell growth-inhibiting gene 46 protein; GIG46, AAT6, ACTSA, MYMY5, actin, aortic smooth muscle; growth-inhibiting gene 46; smooth muscle alpha-actin
0610041G09Rik; a-SMA; AAT6; ACTA2; ACTSA; ACTVS; alphaSMA; GIG46; MYMY5; SMalphaA