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|Tested species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG|
|Storage buffer||PBS, pH 7.6, with 15mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Blocking Assay (BLOCK)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Purity: The precipitin lines against goat anti-mouse whole serum are the same as those against goat anti-mouse IgG, Fc fragment specific when assayed by immunoelectrophoresis at an antigen concentration of 20 mg/ml. No reaction was observed against goat anti-mouse IgA, alpha-chain specific or goat anti-mouse IgM, mu-chain specific.
This antibody is recommended for use in blocking assays, immunohistochemical, immunocytochemical and immunofluorescent applications.
Fluorophore: Fluorescein-5-isothiocyanate (FITC-isomer 1)
Amax = 492 nm; Emax = 520 nm
Fluorophore/Protein: 9.3 ug/mg; 3.5 moles FITC per mole IgG
Restoration and Storage: Store product at 4°C until opened. Restore with 0.55 ml distilled water (2.0 mg/ml after restoration). Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. Product may be stored for up to 6 weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day.
To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of 50% glycerol and store at -20°C
Country of Origin: USA
These immunoglobulins are useful as qualitative or quantitative protein standards and as blocking agents in certain immunodetection methods. Product # 31505 is a highly-purified IgG immunoglobulin from the serum of a non-immunized animal, primarily used as an experimental control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Control; Immunoglobulin Control; Isotype Control; Negative Control