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|Tested species reactivity||Not Applicable|
|Host / Isotype||Human / IgG|
|Storage buffer||PBS, pH 7.6, with 15mg/ml BSA|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Blocking Assay (BLOCK)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Purity: The precipitin lines against goat anti-human whole serum are the same as those against goat anti-human IgG, Fc fragment specific when assayed by immunoelectrophoresis at an antigen concentration of 20 mg/ml. No reaction was observed against goat anti-human IgM, Fc5-mu fragment specific, or goat anti-human IgA, alpha chain specific.
This antibody is recommended for use in blocking assays, immunohistochemical, immunocytochemical and immunoflourescent applications.
Restoration and Storage: Store product at 2-8°C until opened. Restore with 1.1 ml distilled water. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pipette. Product may be stored for several weeks at -70°C as undiluted liquid. After dilution, do not use for more than one day.
To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of 50% glycerol and store at -20°C.
Country of Origin: USA
IgG, a monomer, is the predominant Ig class present in human serum. Produced as part of the secondary immune response to an antigen, this class of immunoglobulin constitutes approximately 75% of total serum Ig. IgG is the only class of Ig that can cross the placenta in humans, and it is largely responsible for protection of the newborn during the first months of life. Because of its relative abundance and excellent specificity toward antigens, IgG is the principle antibody used in immunological research and clinical diagnostics. These immunoglobulins are useful as qualitative or quantitative protein standards and as blocking agents in certain immunodetection methods. 31465 is a highly-purified IgG immunoglobulin from the serum of a non-immunized animal, primarily used as an experimental control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.