|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Storage buffer||PBS with 4mg/ml BSA, sucrose|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 4 publications below|
Allophycocyanin (APC) is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing exceptional quantum yields and molar extinction coefficients.
CD7 (gp40, Leu9) is a member of the immunoglobulin gene superfamily. Its N-terminal amino acids 1-107 are highly homologous to Ig kappa-L chains whereas the carboxy-terminal region of the extracellular domain is proline-rich and has been postulated to form a stalk from which the Ig domain projects. CD7 is expressed on the majority of immature and mature T-lymphocytes, and T cell leukemia. It is also found on natural killer cells, a small subpopulation of normal B cells and on malignant B cells. Cross-linking surface CD7 positively modulates T cell and NK cell activity as measured by calcium fluxes, expression of adhesion molecules, cytokine secretion and proliferation. CD7 associates directly with phosphoinositol 3'-kinase. CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation.
Analyte Specific Reagent
Reactive T cells by flow cytometry distinguish Hodgkin lymphomas from T cell/histiocyte-rich large B cell lymphoma.
MHCD0705 was used in flow cytometry to discuss using the infiltrate to diagnosis B-cell lymphomas.
|Wu D,Thomas A,Fromm JR||Cytometry. Part B, Clinical cytometry (90:424)||2016|
Generation of human acute lymphoblastic leukemia xenografts for use in oncology drug discovery.
MHCD0705 was used in flow cytometry to describe procedures to establish and maintain primary acute lymphoblastic leukemia xenograft panels
|Holmfeldt L,Mullighan CG||Current protocols in pharmacology (68:14.32.1)||2015|
Flow cytometry of ALK-negative anaplastic large cell lymphoma of breast implant-associated effusion and capsular tissue.
MHCD0705 was used in flow cytometry to characterize two cases of anaplastic large cell lymphoma using flow cytometry.
|Wu D,Allen CT,Fromm JR||Cytometry. Part B, Clinical cytometry (88:58)||2015|
Flow cytometry significantly improves the diagnostic value of fine needle aspiration cytology of lymphoproliferative lesions of salivary glands.
MHCD0705 was used in flow cytometry to compare fine needle aspiration cytology with flow cytometry immunophenotyping for the diagnosis of lymphoproliferative processes in the salivary glands.
|Stacchini A,Aliberti S,Pacchioni D,Demurtas A,Isolato G,Gazzera C,Veltri A,Maletta F,Molinaro L,Novero D||Cytopathology : official journal of the British Society for Clinical Cytology (25:231)||2014|