Immunofluorescence analysis of INSR [pY972] was done on 70% confluent log phase MCF7 cells with insulin treatment (100nM for 5 min). The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with INSR [pY972] Rabbit polyclonal Antibody (44800G) at 2µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing membrane localization. Panel e is untreated MCF7 cells. Panel f shows no primary antibody control. The images were captured at 20X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rat, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of the human insulin receptor that contains tyrosine 972, as numbered according to Ebina, et al. (tyrosine 960 according to Ullrich, et al.). The sequence is conserved in mouse and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Biological actions of insulin and IGF-1 are mediated by their respective cell surface receptor tyrosine kinases that regulate multiple signaling pathways through activation of a series of phosphorylation cascades. The insulin receptor. Insulin/IGF-1 binding to the extracellular domain leads to autophosphorylation of downstream target proteins. These two receptors differ in sequence in regions that confer specificity for the designated ligand as well as in certain intracellular signaling domains, resulting in significant differences in the functional consequences of activation of each receptor. Defects in IR are the cause of various insulin resistance syndromes and IGF-1R defects may cause some forms of growth retardation. Both these signaling cascades are also important for the development of cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Novel method demonstrates differential ligand activation and phosphatase-mediated deactivation of insulin receptor tyrosine-specific phosphorylation.
44-800G was used to research a novel method of differential ligand activation and phosphatase-mediated deactivation of insulin receptor tyrosine-specific phosphorylation
|Cieniewicz AM,Cooper PR,McGehee J,Lingham RB,Kihm AJ||Cellular signalling (28:1037)||2016|
Role of protein farnesylation in burn-induced metabolic derangements and insulin resistance in mouse skeletal muscle.
44-800G was used in western blot to elucidate the role of farnesylation in burn-induced metabolic aberration
|Nakazawa H,Yamada M,Tanaka T,Kramer J,Yu YM,Fischman AJ,Martyn JA,Tompkins RG,Kaneki M||PloS one (10:null)||2015|
Hypothalamic prolyl endopeptidase (PREP) regulates pancreatic insulin and glucagon secretion in mice.
44-800G was used in western blot to examine the role of prolyl endopeptidase in glucose metabolism and pancreatic function
|Kim JD,Toda C,D'Agostino G,Zeiss CJ,DiLeone RJ,Elsworth JD,Kibbey RG,Chan O,Harvey BK,Richie CT,Savolainen M,Myöhänen T,Jeong JK,Diano S||Proceedings of the National Academy of Sciences of the United States of America (111:11876)||2014|
|Mouse||Not Cited||Reducing amyloid-related Alzheimer's disease pathogenesis by a small molecule targeting filamin A.||Wang HY,Bakshi K,Frankfurt M,Stucky A,Goberdhan M,Shah SM,Burns LH||The Journal of neuroscience : the official journal of the Society for Neuroscience (32:9773)||2012|
|Rat||Not Cited||Insulin enhances growth hormone induction of the MEK/ERK signaling pathway.||Xu J,Keeton AB,Franklin JL,Li X,Venable DY,Frank SJ,Messina JL||The Journal of biological chemistry (281:982)||2006|