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Western blot analysis from lysates of Jurkat human acute T cell leukemia cell line, HT-2 mouse T cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 0.5 µg/mL of human/mouse/Rat NLRP10/Pynod/NALP10 Monoclonal Antibody (Product # MA5-24260) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. A specific band was detected for NLRP10/Pynod/NALP10 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions.
|Tested species reactivity||Human , Rat , Mouse|
|Host / Isotype||Mouse / IgG2B|
|Immunogen||E. coli-derived recombinant human NLRP10/Pynod/NALP10 Met386-Ile655|
|Storage buffer||PBS with 5% trehalose|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In direct ELISAs, no cross-reactivity with recombinant human (rh) NALP1 (aa 1-323), rhNALP1 (aa 1331-1429), rhNALP2 (aa 1-208), rhNALP3 (aa 3-223), rhNALP3 (aa 540-689), rhNALP5 (aa 469-635), rhNOD1 (aa 830-953), or rhNOD2 (aa 230-450) is observed.
Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
NALP10, a member of the NALP protein family, is reported to have a regulatory role in the innate immune system and forms a large signal-induced multiprotein complex, the inflammasome, which activates the pro-inflammatory caspases, caspase-1 and caspase-5. Unlike other members of the family NALP10 lacks the LRR (leucine rich repeat) region but contains a DAPIN domain and a NACHT domain. Immunoprecipitation reports suggest that NALP10 interacted with ASC, CASP1, and IL-1B and inhibits NF-kB activation, CASP1/caspase-1-dependent IL-1B secretion and apoptosis induced by ASC (PYCARD) in a dose-dependent manner. Expression of NALP10 is enhanced by stimulation with lipopolysaccharide. Experiments with NALP10 suggest that it acts as a multifunctional negative regulator of inflammation and apoptosis. Wide expression of NALP10 is reported with highest levels in heart, brain and skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
NACHT, LRR and PYD domains-containing protein 10, NACHT, leucine rich repeat and PYD containing 10, nucleotide-binding oligomerization domain protein 8, nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 10, NACHT, LRR and PYD containing protein 10, NACHT/LRR/pyrin domain-containing protein 10, neuronal apoptosis inhibitor protein 10, CLR11.1, NALP10, NOD8, PAN5, PYNOD, NACHT, LRR and PYD domains-containing protein 10, leucine rich repeat and PYD containing 10, nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 10
CLR11.1, NOD8, Nalp10, NALP10, 6430548I20Rik, PYNOD, Pynod, Napl10, PAN5