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Western Blot staining of HEK293 lysate overexpressing Human GOLM1 (c-term Myc tagged and loaded in Lane A&C) stained with EB005667 at a concentration of 1 ug/ml (lane A). Lane C was stained with anti-MYC Tag at a dilution of (1/1000). Lane B was loaded with mock-transfected HEK293 cells and stained using PA5-18100 at a concentration of 1 mg/ml. Primary antibody incubation was performed for 1 hour and detection was performed by chemiluminescence.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide sequence (NLLDQREKRNHTL) corresponding to the C-terminus amino acids of GOLM1|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4ug/ml|
|Western Blot (WB)||0.2-1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes proteins synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this gene has been observed to be upregulated in response to viral infection. Alternatively spliced transcript variants encoding the same protein have been described for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
bA379P1.3; C9orf155; epididymis luminal protein 46; FLJ22634; FLJ23608; Golgi membrane protein 1; Golgi membrane protein GP73; Golgi phosphoprotein 2; golgi protein, 73-kD; GOLM1; GOLPH2; GP73; PSEC0257; RP11-379P1.3
bA379P1.3; C9orf155; GOLM1; GOLPH2; GP73; HEL46; PSEC0242; PSEC0257; UNQ686/PRO1326