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Flow cytometric analysis of HeLa cells were stained with mouse Anti-human Insulysin/IDE Monoclonal Antibody (Product # MA5-24064) or isotype control antibodyopen histogram), followed by Allophycocyanin-conjugated Anti-mouse IgG F(ab')2 Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Sf 21-derived recombinant human Insulysin/IDE Met42-Leu1019|
|Storage buffer||PBS with 5% trehalose|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||2.5 µg/10^6 cells|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Reconstitute at 0.5 mg/mL in sterile PBS.
This gene may belong to a protease family responsible for intercellular peptide signalling. Though its role in the cellular processing of insulin has not yet been defined, insulin-degrading enzyme is thought to be involved in the termination of the insulin response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Abeta-degrading protease, insulin protease, insulin-degrading enzyme, insulinase, INSULYSIN